Amino Acid Synthesis by Reversal of the Amino Acid Oxidase Reaction

Amino acid synthesis by reversal of the amino acid oxidase reaction.

Although the intermediate formation of an imino acid is consistent with available data, direct experimental evidence for it is still lacking. The reaction in the presence of air proceeds to completion and is essentially irreversible because of reoxidation of the reduced flavoenzyme by molecular oxygen. Previously, the amino acid oxidase reaction has been studied only in the direction of cr-keto...

Reaction mechanism of flavin dehydrogenation by D-amino acid oxidase

Introduction Since its discovery, D-amino acid oxidase (EC 1.4.3.3, DAAO) has received much attention both from the physiological and biochemical points ofview. The groups of Massey (USA), Curti (1taly), and Yagi (Japan) were mainly involved in its study over the year (1). In spite of these efforts the basic molecular mechanism underlying the reductive half-reaction, i.e. the dehydrogenation st...

INHIBITION OF THE d-AMINO ACID OXIDASE BY BENZOIC ACID

Benzoic acid has been reported to inhibit the oxidation of succinic acid by muscle (l), the oxidation of butyric acid and crotonic acid by liver slices (2), and the oxidation of acetoacetic acid by kidney slices (3). It has also been reported to inhibit the oxygen uptake of liver and kidney slices, diaphragm, minced liver, kidney, and brain (4). In the present work it was found that benzoic aci...

Astudyon D-amino-acid Oxidase

The L-amino acid oxidase of Neurospora.

In 1944 one of us described a n-amino acid oxidase in extracts of Neurospora crassa (1). Except for slight activity against L-glutamate, no oxidation of L-amino acids was observed. Recently a means for inducing the formation of a soluble L-amino acid oxidase by the mold was reported by Bender, Krebs, and Hor0wit.z (2). This is accomplished by reducing the biotin content of the basal medium (3) ...